Monitoring toxic microalgae Ostreopsis (dinoflagellate) species in coastal waters of the Mediterranean Sea using molecular PCR-based assay combined with light microscopy   总被引：1，自引：0，他引：1  

Cecilia Battocchi  Magda Vila  Samuela Capellacci  Albert Reñé  Antonella Penna 《Marine pollution bulletin》2010,60(7):1074-7059

A molecular PCR-based assay was developed and applied to macrophyte and seawater samples containing mixed microphytobenthic and phytoplanktonic assemblages, respectively, in order to detect toxic Ostreopsis species in Mediterranean Sea. The specificity and sensitivity of the molecular PCR assay were assessed with both plasmidic and genomic DNA of the target genus or species using taxon-specific primers in the presence of background macrophyte DNA. The PCR molecular technique allowed rapid detection of the Ostreopsis cells, even at abundances undetectable within the resolution limit of the microscopy technique. Species-specific identification of Ostreopsis was determined only by PCR-based assay, due to the inherent difficulty of morphological identification in field samples. In the monitoring of the toxic Ostreopsis blooms PCR-based methods proved to be effective tools complementary to microscopy for rapid and specific detection of Ostreopsis and other toxic dinoflagellates in marine coastal environments.  相似文献   

Modified sediments and subsurface hydrology in natural and recreated salt marshes and implications for delivery of ecosystem services          下载免费PDF全文

James A. Tempest  Gemma L. Harvey  Kate L. Spencer 《水文研究》2015,29(10):2346-2357

Recreation or restoration of salt marsh through the deliberate removal of flood defences (managed realignment or de‐embankment) is a common practice across Europe and the USA, with potential to enhance delivery of ecosystem services. However, recent research suggests that physical, chemical and ecological processes may be impaired in recreated sites as a result of the modified morphology, sediment structure and hydrology associated with both the restoration process and historic land use. This paper compares physical sediment properties and subsurface water levels recorded in paired natural and de‐embanked (recreated) salt marshes in SE England. Using a combination of statistical and time‐series modelling, significant differences between the natural and recreated marshes are identified. Sediment properties (bulk density, moisture content and organic content) within each marsh were statistically different and imply that de‐embanked sediments are compacted, which may affect subsurface water movement. Analysis of hydrological time series reveals that the de‐embanked salt marsh is characterized by a damped response to tidal flooding with elevated and less variable water levels. This, combined with analysis of hydrographs and hysteresis patterns over individual tidal cycles, suggests that fast, horizontal near‐surface flows enhanced by the relict land surface may play a greater role in drainage of the de‐embanked salt marsh. The importance of hydrological functioning in governing many important physical and biogeochemical processes in salt marshes suggests any modifications would have significant implications for the delivery of ecosystem services. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   

The impact of ditch blocking on the hydrological functioning of blanket peatlands   总被引：1，自引：0，他引：1       下载免费PDF全文

Joseph Holden  Sophie M. Green  Andy J. Baird  Richard P. Grayson  Gemma P. Dooling  Pippa J. Chapman  Christopher D. Evans  Mike Peacock  Graeme Swindles 《水文研究》2017,31(3):525-539

Ditch blocking in blanket peatlands is common as part of peatland restoration. The effects of ditch blocking on flow regimes and nearby water tables were examined in a field trial. After an initial 6‐month monitoring period, eight ditches had peat dams installed 10 m apart along their entire length (dammed), four of these ditches were also partially infilled through bank reprofiling (reprofiled). Four ditches were left open with no dams or reprofiling (open). These 12 ditches and the surrounding peat were monitored for 4 more years. An initial five‐fold reduction in discharge occurred in the dammed and the reprofiled ditches with the displaced water being diverted to overland flow and pathways away from the ditches. However, there was a gradual change over time in ditch flow regime in subsequent years, with the overall volume of water leaving the dammed and the reprofiled ditches increasing per unit of rainfall to around twice that which occurred in the first year after blocking. Hence, monitoring for greater than one year is important for understanding hydrological impacts of peatland restoration. Overland flow and flow in the upper ~4 cm of peat was common and occurred in the inter‐ditch areas for over half of the time after ditch blocking. There was strong evidence that topographic boundaries of small ditch catchments, despite being defined using a high‐resolution Light Detection And Ranging‐based terrain model, were not always equivalent to actual catchment areas. Hence, caution is needed when upscaling area‐based fluxes, such as aquatic carbon fluxes, from smaller scale studies including those using ditches and small streams. The effect of ditch blocking on local water tables was spatially highly variable but small overall (time‐weighted mean effect <2 cm). Practitioners seeking to raise water tables through peatland restoration should first be informed either by prior measurement of water tables or by spatial modelling to show whether the peatland already has shallow water tables or whether there are locations that could potentially undergo large water‐table recoveries.  相似文献   

Erratum to: Along-slope oceanographic processes and sedimentary products around the Iberian margin     

Francisco Javier Hern��ndez-Molina  Nuno Serra  Dorrik A. V. Stow  Estefan��a Llave  Gemma Ercilla  David Van Rooij 《Geo-Marine Letters》2011,31(5-6):547-547

  相似文献   

A Least-Cost Approach to Personal Exposure Reduction     

Gemma Davies  Duncan Whyatt 《Transactions in GIS》2009,13(2):229-246

Concerns over the potential negative health effects from exposure to air pollution have led to interest in assessing personal exposure and finding ways to reduce it. As journey-time exposure accounts for a disproportionately high amount of an individual's total exposure, this article assesses the potential to apply least-cost techniques within a GIS in order to identify paths of lower journey-time exposure. The methodology adopted uses pollution surfaces for PM₁₀ and CO generated by the dispersion model ADMS, with an analysis mask derived from OS MasterMap to create a least-cost surface. Actual routes taken by a cohort of 11–13 year old children on their journeys to school are used to compare observed journey time exposure with the exposure along alternative routes generated using the least-cost path function. While the least-cost approach proved to be successful in defining low exposure routes the ability to scale up this approach is constrained by the amount of editing required to successfully create an analysis mask from OS MasterMap data. Such alternative routes have the potential to assist in promoting safer environmental choices, however, their likelihood of adoption is dependant on a number of social and environmental influences which affect an individual's route choice.  相似文献   

Pliocene�CQuaternary contourites along the northern Gulf of Cadiz margin: sedimentary stacking pattern and regional distribution     

Estefan��a Llave  Hugo Matias  F. Javier Hern��ndez-Molina  Gemma Ercilla  Dorrik A. V. Stow  Teresa Medialdea 《Geo-Marine Letters》2011,31(5-6):377-390

This study reports novel findings on the Pliocene?CQuaternary history of the northern Gulf of Cadiz margin and the spatiotemporal evolution of the associated contourite depositional system. Four major seismic units (P1, P2, QI and QII) were identified in the Pliocene?CQuaternary sedimentary record based on multichannel seismic profiles. These are bounded by five major discontinuities which, from older to younger, are the M (Messinian), LPR (lower Pliocene revolution), BQD (base Quaternary discontinuity), MPR (mid-Pleistocene revolution) and the actual seafloor. Unit P1 represents pre-contourite hemipelagic/pelagic deposition along the northern Gulf of Cadiz margin. Unit P2 reflects a significant change in margin sedimentation when contourite deposition started after the Early Pliocene. Mounded elongated and separated drifts were generated during unit QI deposition, accompanied by a general upslope progradation of drifts and the migration of main depocentres towards the north and northwest during both the Pliocene and Quaternary. This progradation became particularly marked during QII deposition after the mid-Pleistocene (MPR). Based on the spatial distribution of the main contourite depocentres and their thickness, three structural zones have been identified: (1) an eastern zone, where NE?CSW diapiric ridges have controlled the development of two internal sedimentary basins; (2) a central zone, which shows important direct control by the Guadalquivir Bank in the south and an E?CW Miocene palaeorelief structure in the north, both of which have significantly conditioned the basin-infill geometry; and (3) a western zone, affected in the north by the Miocene palaeorelief which favours deposition in the southern part of the basin. Pliocene tectonic activity has been an important factor in controlling slope morphology and, hence, influencing Mediterranean Outflow Water pathways. Since the mid-Pleistocene (MPR), the sedimentary stacking pattern of contourite drifts has been less affected by tectonics and more directly by climatic and sea-level changes.  相似文献   

Along-slope oceanographic processes and sedimentary products around the Iberian margin   总被引：4，自引：2，他引：2  

Francisco Javier Hern��ndez-Molina  Nuno Serra  Dorik A. V. Stow  Estefan��a Llave  Gemma Ercilla  David Van Rooij 《Geo-Marine Letters》2011,31(5-6):315-341

This contribution to this special volume represents the first attempt to comprehensively describe regional contourite (along-slope) processes and their sedimentary impacts around the Iberian margin, combining numerically simulated bottom currents with existing knowledge of contourite depositional and erosional features. The circulation of water masses is correlated with major contourite depositional systems (CDSs), and potential areas where new CDSs could be found are identified. Water-mass circulation leads to the development of along-slope currents which, in turn, generate contourite features comprising individual contourite drifts and erosional elements forming extensive, complex CDSs of considerable thickness in various geological settings. The regionally simulated bottom-current velocities reveal the strong impact of these water masses on the seafloor, especially in two principal areas: (1) the continental slopes of the Alboran Sea and the Atlantic Iberian margins, and (2) the abyssal plains in the Western Mediterranean and eastern Atlantic. Contourite processes at this scale are associated mainly with the Western Mediterranean Deep Water and the Levantine Intermediate Water in the Alboran Sea, and with both the Mediterranean Outflow Water and the Lower Deep Water in the Atlantic. Deep gateways are essential in controlling water-mass exchange between the abyssal plains, and thereby bottom-current velocities and pathways. Seamounts represent important obstacles for water-mass circulation, and high bottom-current velocities are predicted around their flanks, too. Based on these findings and those of a selected literature review, including less easily accessible ??grey literature?? such as theses and internal reports, it is clear that the role of bottom currents in shaping continental margins and abyssal plains has to date been generally underestimated, and that many may harbour contourite systems which still remain unexplored today. CDSs incorporate valuable sedimentary records of Iberian margin geological evolution, and further study seems promising in terms of not only stratigraphic, sedimentological, palaeoceanographic and palaeoclimatological research but also possible deep marine geohabitats and/or mineral and energy resources.  相似文献   

Interaction between seabed morphology and water masses around the seamounts on the Motril Marginal Plateau (Alboran Sea, Western Mediterranean)     

Desir��e Palomino  Juan-Tom��s V��zquez  Gemma Ercilla  Bel��n Alonso  Nieves L��pez-Gonz��lez  V��ctor D��az-del-R��o 《Geo-Marine Letters》2011,31(5-6):465-479

The seabed morphology in the vicinity of the seamounts on the Motril Marginal Plateau (northern Alboran Sea) was investigated using high-resolution (sparker) and very high-resolution (TOPAS) seismic reflection profiles and multibeam bathymetry. The aim of the study was to determine the recent geological processes, and in particular those that control the contourite depositional system associated with the intermediate and deep Mediterranean water masses. Six groups of morphological features were identified: structural features (seamount tops, tectonic depressions), fluid escape-related features (pockmarks), mass-movement features (gullies, slides), bottom-current features (moats, scour marks, terraces, elongated and separated drifts, plastered drifts, confined drifts, sheeted drifts), mixed features (ridges) and biogenic features (including evidence of (dead) cold water corals such as Lophelia pertusa and Madrepora oculata). The main processes controlling the formation of these features are recent tectonic activity and the interaction of Mediterranean water masses with the seafloor topography. Seamounts act as topographic barriers that affect the pathway and velocity of the deep Mediterranean water masses, which are divided into strands that interact with the surrounding seafloor. The influence of the intermediate Mediterranean water mass, by contrast, is restricted mainly to the tops of the seamounts. Sediment instability and fluid-escape processes play a minor role, their occurrence being probably related to seismicity.  相似文献   

Alongshore variation in the rip current hazard at Pensacola Beach, Florida   总被引：2，自引：1，他引：1  

Chris Houser  Gemma Barrett  Daniel Labude 《Natural Hazards》2011,57(2):501-523

Many drowning and near drownings at Pensacola Beach, Florida are attributed to rip currents, the strong seaward-flowing currents that extend from the shoreline to the line of breakers and sometimes beyond. While surf forecasts assume that the rip hazard is uniform alongshore and that the (erosion) rips are ephemeral features, evidence is presented to suggest that the rip hazard at Pensacola Beach is not uniform alongshore. Rather the rip current “hotspots” develop as a consequence of an alongshore variation in the surf similarity parameter and nearshore state on the order of ~1,450 m. The variation is forced by transverse ridges on the inner shelf that force wave refraction and focusing at the ridge crests. This creates a more dissipative, rhythmic bar and beach morphology at the ridges and rougher surf. Between ridges, where wave heights and periods are smaller and the outermost bar is forced closer to the shoreline, the nearshore is in a (more reflective) bar and rip state during red flag conditions. Drownings between 2000 and 2009 are shown to be clustered between transverse ridges and in the years following a hurricane or tropical storm (2000–2003 and 2005–2008) when the bar and rip morphology first develops as the shore face recovers. This continues until the innermost bar attaches to the beach face unless the bar system is reset by another tropical storm or hurricane. It is argued that the rip hazard is dependent on the alongshore covariation of the environmental forcing with the individual and group behavior in both time and space, even on what appears to be a relatively uniform beach environment.  相似文献   

Esterification of vertebrate-like steroids in the eastern oyster (Crassostrea virginica)     

Gemma Janer  Sonia Mesia-Vela  Margy L. Wintermyer  Keith R. Cooper  Fred C. Kauffman  Cinta Porte 《Marine environmental research》2004,58(2-5):481

The esterification of two model vertebrate steroid hormones – estradiol (E2) and dehidroepiandrosterone (DHEA) – was studied in the oyster Crassostrea virginica. The activity of acyl-CoA:steroid acyltransferase was characterized in microsomal fractions isolated from oyster digestive glands. The apparent K_m and V_max values changed with the fatty acid acyl-CoA used (C20:4, C18:2, C18:1, C16:1, C18:0 or C16:0), and were in the range of 9–17 μM, and 35–74 pmol/min/mg protein for E2, and in the range of 45–120 μM, and 30–182 pmol/min/mg protein for DHEA. Kinetic parameters were also assessed in gonadal tissue. The enzyme saturated at similar concentrations, although conjugation rates were lower than in digestive gland. Preliminary data shows that tributyltin (TBT) in the low μM range (1–50) strongly inhibits E2 and DHEA esterification, the esterification of E2 being more sensitive to inhibition than that of DHEA. Overall, results indicate that apolar conjugation occurs in oysters, in both digestive gland and gonads, at a very similar rate to mammals, suggesting that this is a well conserved conjugation pathway during evolution. Esterification, together with other mechanisms, can modulate endogenous steroid levels in C. virginica, and might be a target for endocrine disrupters, such as TBT.  相似文献